Transcriptional regulation: Kamikaze activators

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Transcriptional regulation: Kamikaze activators

Transcription factors are often targeted for rapid degradation by the ubiquitin-proteasome system. Recent evidence points to a correlation between the potency and instability of transcriptional activators, suggesting a possible direct role for ubiquitin-dependent proteolysis in transcriptional activation.

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A longstanding quest of developmental biology has been to understand the molecular switches that determine which sets of genes are expressed in selected differentiated cell types. Since regulation of gene expression is predominantly at the level of mRNA synthesis (reviewed in Ref. 1), the problem initially becomes one of defining the protein factors that establish and maintain a gene in a trans...

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Transcriptional activators in yeast

Eukaryotic transcription activation domains (ADs) are not well defined on the proteome scale. We systematicallly tested approximately 6000 yeast proteins for transcriptional activity using a yeast one-hybrid system and identified 451 transcriptional activators. We then determined their transcription activation strength using fusions to the Gal4 DNA-binding domain and a His3 reporter gene which ...

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Transcriptional activators need to be modulated and eventually switched off after the initial event that triggers their activation. Here, we discuss how ubiquitination of activators and their proteasome-mediated turnover are crucial steps in this process.

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Transcriptional regulatory mechanisms are fundamentally similar in eukaryotic organisms [1,2]. Components of the RNA polymerase II (Pol II) machinery are highly conserved and, in some cases, functionally interchangeable. Transcriptional activators with similar DNA-binding specificities are present from yeast to human, and acidic activation domains stimulate transcription across a wide range of ...

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ژورنال

عنوان ژورنال: Current Biology

سال: 2000

ISSN: 0960-9822

DOI: 10.1016/s0960-9822(00)00462-0